WPM | McCown's Woody Plant Basic Medium

Item number: MD103

Standard medium for many woody plant species.

Contains macro and micronutrients and vitamins such as described by Lloyd & McCown (1980). It is produced by using a corrected formula according to Owen & Miller (1992) with 100 µM manganese(II)-sulfate. Thereby its concentration is adjusted to the formulation which is also called later as the popular medium "WPM" (Smith and McCown, 1983).

Category: McCown's Woody Plant

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Standard medium for many woody plant species.

Contains macro and micronutrients and vitamins such as described by Lloyd & McCown (1980). It is produced by using a corrected formula according to Owen & Miller (1992) with 100 µM manganese(II)-sulfate. Thereby its concentration is adjusted to the formulation which is also called later as the popular medium "WPM" (Smith and McCown, 1983).


References:

Lloyd, G & B McCown. 1980. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Combined Proceedings, International Plant Propagators' Society 30:421-427
Owen, H. R. and Miller, A. R. (1992) ?An examination and correction of plant tissue culture basal medium formulations?, Plant Cell, Tissue and Organ Culture.
Smith, M. A. L. and McCown, B. H. (1983) ?A Comparison of Source Tissue for Protoplast Isolation from Three Woody Plant Species?, Plant Science Letters, 28(2), pp. 149?156.

Achtung!

GHS symbol 07 Exclamation mark
H315: Verursacht Hautreizungen
H319: Verursacht schwere Augenreizung.
H335: Kann die Atemwege reizen.
H412: Schädlich für Wasserorganismen, mit langfristiger Wirkung.
   
P273: Freisetzung in die Umwelt vermeiden.
P280: Schutzhandschuhe / Augenschutz / Gesichtsschutz tragen.
P305+P351+P338: BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen.
Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.
Aussehen: Pulver
Aggregatzustand: fest
Lagerung: 2 - 8 °C

Zusammensetzung

Makronährstoffe
Kaliumdihydrogenphosphat KH2PO4 170,000 mg/l
Calciumnitrat (wasserfrei) Ca(NO3)2 386,330 mg/l
Calciumchlorid CaCl2 72,500 mg/l
Kaliumsulfat K2SO4 990,000 mg/l
Ammoniumnitrat NH4NO3 400,000 mg/l
Magnesiumsulfat MgSO4 180,540 mg/l
Mikronährstoffe
Kupfer(II)Sulfat Pentahydrat CuSO4· 5 H2O 0,250 mg/l
Eisenchelat FeNaEDTA 36,700 mg/l
Borsäure H3BO3 6,200 mg/l
Mangan(II)Sulfat Monohydrat MnSO4 · H2O 16,900 mg/l
Natriummolybdat Dihydrat Na2MoO4· 2 H2O 0,250 mg/l
Zinksulfat Heptahydrat ZnSO4· 7 H2O 8,600 mg/l
Vitamine
Glycin 2,000 mg/l
myo-Inositol 100,000 mg/l
Nikotinsäure 0,500 mg/l
Pyridoxin HCl 0,500 mg/l
Thiamin HCl 1,000 mg/l

The newest safety datasheets or certificate of analysis (CoA) can get requested by using our contact form. They also can be requested during the order process or directly per mail via info@dephyte.com.

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