Venus Fly Trap (Dionaea) Shoot Proliferation Medium A

Item number: MD166

This medium is used in the in vitro multiplication stage for stimulating the proliferation of Dionaea species (venus flytrap). Originally developed for establishment of a new protocol for in vitro-Mikropropagation of Dionaea muscipula Ellis (Jang, Kim and Park, 2003).

Category: Carnivorous Plants

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Description

Suitable for the in vitro multiplication stage of Dionaea species (syn. Venus Flytrap). This medium is a modification of the MS-Medium (Murashige and Skoog, 1962). The macroelement strength as been reduced to 1/3, and the media is additionally supplemented with 2.3 µM Kinetin. This medium has been developed during the establishment of a micropropagation protocol for Dionaea muscipula Ellis (Jang, Kim and Park, 2003). According to this study, the optimum pH for shoot proliferation and elongation is pH 5.5, whereas lower pH values seem not to be suitable.  By subcultivation every 8 weeks, a high survival rate in the following stages and the generation of several hundret explants from one single explant within a year has been reported.

References

Jang, G.-W., Kim, K.-S. and Park, R.-D. (2003) ?Micropropagation of Venus fly trap by shoot culture?, Plant Cell, Tissue and Organ Culture. Kluwer Academic Publishers, 72(1), pp. 95?98. doi: 10.1023/A:1021203811457.
Murashige, T. and Skoog, F. (1962) ?A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures?, Physiologia Plantarum. Blackwell Publishing Ltd, 15(3), pp. 473?497. doi: 10.1111/j.1399-3054.1962.tb08052.x.

 

Safety informations

Achtung!

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H315: Verursacht Hautreizungen
H319: Verursacht schwere Augenreizung.
H335: Kann die Atemwege reizen.
H412: Schädlcih für Wasserorganismen, mit langfristiger Wirkung.
   
P273: Freisetzung in die Umwelt vermeiden.
P280: Schutzhandschuhe / Augenschutz / Gesichtsschutz tragen.
P305+P351+P338: BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen.
Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.

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