NLN-20 Medium

Item number: MD143

For the generation and culture of haploid plants. Contains macroelements, microelements, vitamins, amino acids and sucrose (20 %) such as described by Lichter (1981).

Category: NLN Medium

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Description

The original composition of the NLN medium was described by Nitsch & Nitsch (1967). In 1981 the composition was modified to a 13 % sucrose containing medium (NLN-13) by Lichter (1981). The medium was developed for the isolation of microspores from Brassica napus. The microspores were used to generate haploid plants (Lichter, 1982).
In this medium the source of nitrate is provided by Potassium nitrate. L-glutamine, L- serine and glutathione serves as sources for amino acids.

Powder, solve in water. Dosage: 2.33 g/l

References:

Nitsch, C & JP Nitsch. 1967. The induction of flowering in vitro in stem segments of Plumbago indica L. Planta 72(4): 355-370
Lichter, R. 1981. Anther culture of Brassica napus in a liquid culture medium. Zeitschrift für Pflanzenphysiologie 103(3): 229-237
Lichter, R. 1982. Induction of haploid plants from isolated pollen of Brassica napus. Zeitschrift für Pflanzenphysiologie 105(5): 427-434

Safety informations

Achtung!

GHS symbol 07 Exclamation mark
H315: Verursacht Hautreizungen
H319: Verursacht schwere Augenreizung.
H335: Kann die Atemwege reizen.
H412: Schädlich für Wasserorganismen, mit langfristiger Wirkung.
   
P273: Freisetzung in die Umwelt vermeiden.
P280: Schutzhandschuhe / Augenschutz / Gesichtsschutz tragen.
P305+P351+P338: BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen.
Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.

Specifications

Aussehen: Pulver
Aggregatzustand: fest
Lagerung: 2 - 8 °C

Zusammensetzung

Makronährstoffe
Calciumnitrat Tetrahydrat Ca(NO3)2 · 4 H2O 500,000 mg/l
Kaliumdihydrogenphosphat KH2PO4 125,000 mg/l
Kaliumnitrat KNO3 125,000 mg/l
Magnesiumsulfat MgSO4 61,000 mg/l
Mikronährstoffe
Cobalt(II)Chlorid Hexahydrat CoCl2 · 6 H2O 0,025 mg/l
Kupfer(II)Sulfat Pentahydrat CuSO4 · 5 H2O 0,025 mg/l
Eisenchelat FeNaEDTA 36,700 mg/l
Borsäure H3BO3 10,000 mg/l
Mangan(II)Sulfat Monohydrat MnSO4 · H2O 18,950 mg/l
Natriummolybdat Dihydrat Na2MoO4· 2 H2O 0,250 mg/l
Zinksulfat Heptahydrat ZnSO4 · 7 H2O 10,000 mg/l
Vitamine
Glycin 2,000 mg/l
myo-Inositol 100,000 mg/l
Nikotinsäure 5,000 mg/l
Pyridoxin HCl 0,500 mg/l
Thiamin HCl 0,500 mg/l
Folsäure 0,500 mg/l
Biotin 0,050 mg/l
Aminosäuren
L-Glutamin 800,000 mg/l
L-Serin 100,000 mg/l
Gluthation 30,000 mg/l
Sonstige
Saccharose 200.000 mg/l



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